Acousto-optical method of blood typing: the comparison of application of anti-A and anti-B monoclonal antibodies with standard hemagglutinating sera

The aim of the article is a comparison of resolution of the acousto-optical method for blood typing for two types of reagents: monoclonal antibodies and standard hemagglutinating sera. Materials and Methods: The influence of the concentrations of reagents (monoclonal antibodies and standard hemagglutinating sera), of blood sample, which is to be tested, as well as of the duration of the ultrasonic action on the biological object upon the resolution of acousto-optical method were investigated. The peculiarity of this work is the application of digital photo images processing by pixel analysis previously proposed by the authors. Results: The optimal experimental conditions to obtain maximum of the resolution of the acousto-optical method were found, it creates the prerequisites for a reliable blood typing. Conclusions: The present paper is a further step in the development of acousto-optical method for determining human blood groups.