Chromosome aberration analysis by the method of comparative genomic hybridization in patients with chronic gastritis and gastric cancer

To carry out the cytogenetic study of chromosome aberrations in epithelial tissue of the stomach, the patients were divided into 2 groups. Group I comprised 24 patients with chronic gastritis (12 patients with metaplasia and dysplasia and 12 patients without morphological changes in the epithelium). Group II consisted of 10 patients with histologically verified intestinal-type gastric cancer. Chromosome aberrations were detected in 9 of the 12 patients (75 %) having no morphological changes in the epithelium (15q11.2-q26 deletion was found in 66 %). In patients with epithelial dysplasia, amplification of the chromosomal region 3р12-р23 was most frequently found (55,5 %). The cytogenetic analysis showed the evidence of chromosome aberrations in all studied epithelial samples, while in cells of chronic inflammation focus, aberrations were detected in 70 % of patients. Deletions in chromosomal regions 19p13.2, 19q13.1-q13 and 18q12-q21 were frequently observed in tumor-adjacent epithelial tissue (30 %), while in tumor cells, the frequent chromosomal aberrations were deletions in chromosomal regions 5q12-q23 (30 %) and 18q11.2-q21(30 %) as well as amplifications 20p11.2-p13 (20 %).