The analysis of effectiveness of double-stranded rna-suppressor of proline dehydrogenase gene for elevation of sunflower (Helianthus annuus L.) tolerance to water deficit and salinity

Perspective of using of strain LВА4404 harboring plasmid pBi2E with double-stranded RNA-suppressor, which was prepared on basis of Arabidopsis ProDH1 gene, for the elevation of sunflower ( Helianthus annuus L.) tolerance to water deficit and salinity was estimated. The transgenic regenerants that maintained stress pressure of lethal doses of mannitol (0.4M) or sea water salts (2,0%) demonstrated the elevated level of free proline under stress conditions and its decline under recovery period. These data declare that ProDH gene confer the osmotolerance of sunflower.