Molecular genetic analysis for fusarium species diversity in the winter triticale (Xtriticosecale Wittm. ex A. Camus)

Fusarium is a large genus of fungi and is of interest primarily because numerous species are important plant pathogens. Fusarium can infect about 200 species of cultivated crops and lead to significant yield losses. In this regard, it is necessary to diagnose plants to detect fungi of the Fusarium genus and determine the phytopathogenic load in crops in order to identify a latent infection. The aim of this study is to test developed primers on fungi DNA collection and winter triticale. In this research, the developed primers were tested on 24 samples of fungi, obtained from different collections and on 50 samples of winter triticale from RSAU-MTAA named after K.A. Timiryazev. Primers and probes were selected and tested to determine belonging to the genus Fusarium by real-time polymerase chain reaction (qPCR). There were no false-positive results in the analysis of other types of fungi and related microorganisms. Analytical sensitivity of the developed oligonucleotides isn’t less than 15 copies of plasmid with the target DNA region per qPCR reaction. Diagnostic kit was tested using various Real-Time PCR systems. The triticale samples were tested for the presence of F. avenaceum, F. poae, F. culmorum, F. oxysporum, F. sambucinum. The greatest defeat by Fusarium was in varieties: PRAG522, PRAG507, PRAG 519, OGM-1. The results of the study could be used for screening diagnostic tests.