Rat blood plasma biochemical parameters during the large wax moth larvae extract oral administration Galleria mellonella L. (Lepidoptera: Pyralidae)

The search for alternative sources of biologically active substances is the task of biomedicine, animal husbandry and other industries. The purpose of research is to study the effect of the extract of the larvae of the great wax moth (BWM) Galleria mellonella L. on the biochemical parameters of the blood plasma of rats. To study the role of G. mellonella products, an analysis of biochemical parameters reflecting the general condition against the background of the Ultimate Swimming test was carried out. The study was conducted on two groups of laboratory rats: the experimental group was given an alcoholic extract of G. mellonella larvae (n=7), the control group was given a 40 % water-alcohol solution (n=6). The rats were matched by the method of pair-analogues by weight and sex. In the study of biochemical parameters of blood, it was found that the swimming time of rats in the Forced swimming test at the end of the experiment increased by 22 times (p≤0.05) when drinking the extract of G. mellonella larvae. The introduction of a 40 % extract from native larvae of G. mellonella led to a significant increase in the concentration of malondialdehyde relative to the control group without the average values going beyond the physiological norm. With the introduction of an alcoholic extract, the oxidation processes start faster. From the 4th week of the experiment, in the experimental group of rats in the blood, there was a tendency for the stability of the glucose level within the range of 4.3-5.5 mmol/l, in the control, the tendency to increase the level by the end of the experiment was 4.7-5.0 mmol/l. There is more pyruvic acid in the blood of experimental rats, that is, the oxidation processes are faster, the utilization (mobilization) of glucose is also more intensive. The concentration of cortisol in rats of the experimental group did not change during the entire experiment, which also confirms the level of adaptation of animals to the stress factor. Thus, the alcoholic extract of G. mellonella larvae increases physical endurance and has a faster effect on carbohydrate (energy) metabolism.