Numerical estimation of distribution uniform of molecular colonies for Dpcr results image analysis

Molecular colony technique is one of the sensitive methods of genetic analysis. This technique was implemented on a planar microchip device with array of reaction chambers. The errors of analysis related to the distribution of molecular colonies in the reaction chamber are discussed. An approach based on order statistics is described in the paper. It provides two estimates of the measure of uniform distribution, that are analogues of basic concepts in statistics: point maximum likelihood estimates and interval estimates. Usefulness of this approach was illustrated by analysis of images, gets with cytokeratin’s transcripts (CK-19) determination in sample.