The genus Pseudomonas sp. bacteria effect on triticosecale growth and productivity

The aim of research is to study the effect of bacteria of the genus Pseudomona ssp. on growth rates and grain productivity of triticale in the conditions of the Vologda Region. Tasks: assessment of morphometric, physiological parameters of plants, as well as the structure of the yield of triticale. The study was carried out as part of a small-plot field experiment, which was carried out in 2020 at the Federal State Budgetary Institution of Sciences Vologda Research Center of the Russian Academy of Sciences (VolRC RAS). The object of research is spring triticale variety Dobroe, which is approved for use in the North-West Region of the Russian Federation. The Pseudomona ssp. GEOT18 used in the study was isolated by the staff of the Yaroslavl State University named after P.G. Demidov from the internal tissues of stem-root tuberoids of generative specimens of meat-red palm root (Dactylorhiza incarnata (L.) Soó). The experiment was repeated 3 times, the area of the accounting plot was 2 m2. The sowing of the crop was done manually, in accordance with the accepted seeding rates - 4.5 million germinating seeds/ha. Before sowing, the seeds were divided into two parts: one was placed in a suspension of an overnight culture of the bacterial strain for 30 min (experimental group), the other in water (control group). In the phase of the beginning of tillering, the plants were sprayed with a solution of the suspension until droplets of fine dew appeared on the leaves (dilution of the suspension of the night culture of the strain in a ratio of 1:20). There was an increase in the wet and dry weight of the experimental plants relative to the control ones up to 41.9 and 35.9 %, respectively, and there was also an increase in the total leaf surface of the plants of the experimental group to 53.3 %. The concentration of photosynthetic pigments in the leaves of experimental plants is somewhat lower than the control. The grain productivity of the experimental triticale plants is higher than the control by 16.8 %, which is due to a slight increase in the weight of the caryopsis and an increase in the number of caryopses in the ear by 18.8 %.