The dispersion of mono- and mixed Alcaligenes faecalis 2 and Rhodococcus ruber GT 1 biofilms

The dispersion of mono- and mixed biofilms Al. faecalis 2 and R. ruber gt 1 with nitrile hydrolyzing activity was studied. Dispersion was assessed taking into account two parameters: 1) different age of the biofilm; 2) the exposure time of the grown biofilm in a physiological solution without nutrients. It was shown that the 7-days monofilms of the studied strains are the least stable, the dispersion from which exceeds that of the mixed biofilm by an order of magnitude or more. When comparing the absolute number of migrated cells, it was shown that R. ruber gt 1 cells migrate from monofilm much more intensively than from mixed cells. In a mixed culture, the 4-days old biofilms of Al. faecalis 2 and R. ruber gt 1 are most susceptible to destruction, which corresponds to the moment of the most intensive adhesion of the satellite microorganism (R. ruber gt 1) to the primary colonizer (Al. faecalis 2). It is shown that with further cooperative growth comes the phase of adaptation, which is expressed in the formation of a durable mixed biofilm, which makes it possible to effectively use them in biotechnological processes.