Two-component quantitative PCR analysis

The methodical peculiarities were considered and a method of modeling of two-component PCR analysis was proposed. A comparison of three ways of threshold cycle determination at two-component PCR analyses was performed. The dependencies of measurement errors on account of the threshold cycles difference of measuring and neighboring channels were determined. The advantage of two-component signal approximation offered by us in earlier improved model on the base of sigmoid function with 6 parameters is shown.