Genotypical variability in progeny of transgenic interspecific hybrid of tomato. Communication II. Identification of alien DNA intergration site
Автор: Chesnokov Yu.V., Bocharnikova N.I., Esaulova L.V., Yunusov Z.R.
Журнал: Сельскохозяйственная биология @agrobiology
Рубрика: ДНК-технологии, трансгенез, молекулярное маркирование
Статья в выпуске: 1 т.47, 2012 года.
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It was done the molecular-genetic analysis of 19 forms of transgenic genotypes of different progenies and two parents forms of interspecific tomato hybrid Lycopersicon esculentum L. (cv. Fakel) ½ Solanum pennellii Cor. The difference was revealed between lengths of obtained PCR fragments. Alignment of primary structures of PCR amplicons allowed to determine exact site of integration of alien exogenous DNA in genome of transgenic plants. Based of obtained data it was assumed that integration was done by homologous recombination by triplet AGG from 5' end of integrated in genome fragment and by non-homologous recombination in unidentified 3' end nucleotide site.
Interspecific hybrid of tomato, alien exogenous dna, integration site
Короткий адрес: https://sciup.org/142133266
IDR: 142133266