Genotypical variability in progeny of transgenic interspecific hybrid of tomato. Communication II. Identification of alien DNA intergration site

It was done the molecular-genetic analysis of 19 forms of transgenic genotypes of different progenies and two parents forms of interspecific tomato hybrid Lycopersicon esculentum L. (cv. Fakel) ½ Solanum pennellii Cor. The difference was revealed between lengths of obtained PCR fragments. Alignment of primary structures of PCR amplicons allowed to determine exact site of integration of alien exogenous DNA in genome of transgenic plants. Based of obtained data it was assumed that integration was done by homologous recombination by triplet AGG from 5' end of integrated in genome fragment and by non-homologous recombination in unidentified 3' end nucleotide site.