Histological dyes based on nettle (Urtica dioica) and rowan berries (Sorbus aucuparia) extracts

Histological dyes were obtained from water extracts of nettle leaves and Rowan berries and their staining properties were studied. It was shown that new dyes are cytoplasmic and can be combined with nuclear dyes, for example, with the recently reported dye based on chokeberries. The recipes of dye preparation and staining procedures are simple and cheap and can be used for staining of histological specimens of different organs. Interesting results were obtained after staining of various brain structures. Techniques allow good visualization of nerve fibers and blood vessels and can be used for the study of the brain myelo- and angioarchitecture.