Histological study of the carotid body: A new ultraviolet radiation method

The aim of the study is to create and test a method for identifying the carotid body (CB) parenchyma for light microscopy using ultraviolet radiation. Materials and Methods. We studied 5 fragments of the common carotid artery (CCA) bifurcation obtained during carotid endarterectomy in elderly and senile patients. After excision, the fragment was placed in 10 % buffered formalin. Within 20 min, the material was delivered to the pathology department, where it was placed under a UV light box, 405 nm wavelength, with a rotating tray. As a result, a bright green glow was observed in the CCA bifurcation area. The luminous area was exsected with a scalpel and tweezers. Then, layer-by-layer histologic specimen (5 μm thickness) were made on a rotary microtome (routine technique, Masson‘s trichrome stain). Results. A method for detecting the CB parenchyma for light microscopy using ultraviolet radiation was developed, tested and patented. It allowed for precise determination of CB parenchyma areas. The entire organ histostructure was clearly determined on all histological specimen. This reaction makes the bright green glow visible to the naked eye, eliminating the need for magnifying devices. Conclusions. The new approach can significantly simplify and speed up the process of SB examination, and improve the quality of histological materials.