Multiplex PCR-based identification of potato genotypes as donors in breeding for resistance to diseases and pests

The breeding of potatoes with the traditional technology of hybridization and selection of individual plants is a time-consuming process. The use of DNA markers linked to genes underlying resistance to diseases and pests can significantly improve the efficiency of the selection of valuable genotypes in the early stages of breeding process. The aims of the work were i) screening of potato genetic resources from the VIR collection (Vavilov All-Russian Institute of Plant Genetic Resources, St. Petersburg) for the presence of genes encoding resistance to cyst-forming nematodes, potato wart, potato viruses X and Y (PVX and PVY) by the multiplex PCR method; ii) evaluation of the effectiveness of molecular markers for the identification of potato genotypes resistant to the golden nematode, potato wart and PVY. A total of 90 accessions from the VIR collection were studied, among them the cultivated potatoes from two subspecies, the S. tuberosum subsp. chiloense (native varieties of Chile) and S...