Immunogenic properties of recombinant attenuated strain Salmonella enteritidis Е23 carrying DNA-vaccine coding for HIV-1 polyepitope СTL immunogen

The recombinant attenuated strain Salmonella enteritidis E23/ pcDNA-TCI which carrying DNAvaccine containing gene of CTL-polyepitope HIV-1 immunogen under the control of CMV promoter was constructed. The strain was used for perrectal immunizations of BALB/c mice. Detected after immunization in sera of immunized mice HIV-specific antibodies were demonstrated to recognize both recombinant and native HIV-1 antigens. Double immunization was more effective compared to unitary injection. The results obtained showed that recombinant attenuated Salmonella enteritidis E23/pcDNA-TCI could be perspective for delivery the candidate HIV-1 DNA vaccine.