Intensity of biofilm formationby rhizobacterial strains pseudomonas pseudoalcaligenes

This paper examines the ability of soil rhizobacteria associated with cultivated agricultural plants to form biofilms in vitro.The objective of this study is to examine the activity of biofilm formation of Lycopersicon esculentum Mill. root system by Pseudomonas pseudoalcaligenes rhizobacterial strains depending on the phase of plant vegetation.Materials and Methods. During each phase of the examined plant vegetation 50 root washout samples were selected. They were analyzed for species and quantitative composition; dominant strains of rhizobacteria were determined. Further modeling of biofilm formation by rhizobacterial strains on Petri dishes was carried out. The strains were stained with crystal violet and immersion oils. The biofilm formation activity was examined by a static method using 96-well plates in which the rhizobacteria were incubated. Then, the biofilms obtained were stained with gentian violet. The results were interpreted spectrofluorimetrically according to the staining intensity...