A study of chromosome aberrations and markers of oxidative stress (malondialdehyde, plasma total antioxidant activity) among females residing in territories contaminated with radionuclides as a result of the Chernobyl accident

The goal of the study was to evaluate cytogenetic assay results and markers of oxidative stress (malonic dialdehyde, plasma antioxidant activity) in women living in territories with different levels of radioactive contamination, as well as those resettled in 1991 from areas recommended for resettlement in "clean" areas. Cytogenetic analysis in peripheral blood lymphocytes was performed according to a standard procedure for 200 women. The controls women (54) were living in Moscow and Obninsk. Malondialdehyde (MDA) was determined in the presence of thiobarbituric acid by means of photometry. The plasma total antioxidant activity (AOA) was measured in the trolox system, ABAP-fluorescein (2,2'-azobis(2-methylpropionamidine)). It was shown that the frequency of chromosomal exchanges in groups of women permanently residing in areas with different levels of contamination did not differ from each other, averaging 0.16 per 100 cells, but exceeds by 4.5 times the same index in the control group (p=0.01). Proportion of individuals with dicentrics in these two groups was of similar magnitude 19% and 15% (p=0.6). In the case of migrants, the percentage of women with dicentricks (53%, p