The research of water extract larch Siberian bark antibacterial activity

Автор: Krotova I.V., Grodnitskaya I.D., Kuzina A.N., Kondakova O.E., Shishkina I.V.

Журнал: Вестник Красноярского государственного аграрного университета @vestnik-kgau

Рубрика: Биологические науки

Статья в выпуске: 5, 2017 года.

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The problem of utilization of timber processing and timber cutting waste has not lost its relevance for many decades. It is known that the bark of Sibe-rian larch has bactericidal properties. One of possi-ble applications of using biocidal activity of water extracts of Siberian larch bark is the creation of textile materials with bactericidal and fungicidal properties. The researches of the painting ability of water extracts of Siberian larch bark conducted earlier by the authors of the present study showed that their application in various extent of cultivation is expedient for coloring of polyamide fibers. Thus the scale of beige-brown tones especially demand-ed in hosiery production turns out. In this regard the purpose of the real research was studying of bacte-ricidal activity of water extracts of bark of a Siberian larch depending on their concentration and storage period, and also polyamide fibers painted by them. As test objects museum cultures of conditionally pathogenic bacteria of Pseudomonas aeruginosa HTCC 27885, Klebsiella pneumonia T 904, Esche-richia coli ATCC 39/21141, Staphylococcus aureus ATCC 25922, as well as strains of saprotrophic bacteria Pseudomonas sp., Bacillus subtilis, Bacil-lus amyloliquefaciens were used as test objects. Opportunistic bacteria (hazard class 3-4) are able to cause septic sequel and are highly resistant to many disinfectants and antibiotics. These bacteria are sanitary-indicatory microorganisms and are often found in complications of various diseases in clinics. Saprotrophic bacteria have antagonistic activity to many microorganisms (for example, to phytopathogenic ones). They are often found in soil, water, on food in large numbers, are capable to cause damage. It is shown that not only extracts, but also dries and concentrate polyphenolic Siberi-an larch bark complex, diluted with distilled water to maximum possible concentration, inhibits the growth of all tested bacteria tested for 72 hours. The shelf life of investigated water extracts influ-ences their bactericidal activity. It was found out that after 120 days it remains only in relation to two of four studied conditionally pathogenic bacteria test cultures (Escherichia coli, Staphylococcus aureus). With respect to saprotrophic bacteria, biocidal activity of the extracts of Siberian larch bark does not change. The polyamide fiber dyed by studied extracts, exhibits bactericidal activity only towards Bacillus amyloliquefaciens culture. And this property is retained after five washes. And this property remains and after five washings that al-lows to recommend the processed polyamide threads as raw materials for production of hosiery with antimicrobic properties.

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Larch bark, opportunistic bacteria, bactericidal activity, polyamide fiber, extraction, saprotrophic bacteria

Короткий адрес: https://sciup.org/140224204

IDR: 140224204

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