A Study of Dimethyl Sulfoxyde Cytotoxicity by MTT-test on L929 Cell Culture

The study objective was to investigate the cytotoxicity of dimethyl sulfoxide on the cell culture of the murine fibroblasts L929. The cytotoxicity was studied by the standard procedure of MTT-test. The сells were incubated during 24 hours. Then dimethyl sulfoxide in concentrations from 1 to 0,0625% (by titration 1 : 2) was added. The standard medium without dimethyl sulfoxide was added in equivalent amount as passive control. The exposition time of the studied substances was 48 hours. All experiments were repeated three times. It was found that dimethyl sulfoxide has cytotoxic activity on the cell culture of the murine fibroblasts L929 after 48 hours of incubation at 1 and 0,5% concentration. This fact should be taken into consideration if dimethyl sulfoxide is used as solvent for nonpolar lipophilic substances testing on the cell culture of the murine fibroblasts.