The research of PMMA bonding with elastomer (Sylgard-184, silastik T-4) for microfluidic devices

Irreversible bonding of PMMA with PDMS Sylgard-184 and silastic T-4 for microfluidic devises may be created by silane chemistry. Silastic T-4 usage gives an opportunity to silanise only PMMA to seal plates. For Sylgard-184 application the treatment of both plates is necessary. Bonding stability was tested by cyclic (45 cycles) temperature changing between 60 and 95 °С. It was experimentally shown that silastic T-4 didn’t have an influence on PCR. So this material may be used in analytical devices invention for molecular diagnosis.