Study osteogenic properties of blood plasma fractionation

Purpose. To clear some conditions for realization of the phenomenon of the influence of blood plasma fraction injection on reparative osteogenesis depending on the dose and recipient animal’s regenerative status. Materials and Methods. The influence of blood fractionated plasma injections on reparative osteogenesis depending the dosage and initial recipient’s regenerative status investigated in an experimental study. Blood taking performed in experimental animals (dogs) in the distraction period during leg bone osteosynthesis according to Ilizarov. Plasma fractionation made by original technique. Laboratory mice subjected to rib osteotomy served as recipients. A single injection of fraction within the doses from 10 to 60 mg/kg made before and after surgery. Morphologic and biochemical investigations of blood and tissues performed 10 days after surgery. Results. Blood fractionated plasma found to have both osteoinductive and inhibiting properties. The fraction dose and recipient organism’s metabolic status at the time of injection influence the impact intensity and direction. Conclusion. The results evidence promising use of blood preparations in order to optimize reparative osteogenesis.