The research of acute inhalation toxicity of ozone and air mixture

The research objective was studying acute inhalation toxicity of ozone and air mixture and giving the definition of its class of toxicity. The object of the research was ozone and air mixture. A portable barrier-type ozonizer with ozone capacity of 3.6 g / h was chosen as the source of ozone. The test procedure was carried out according to the standard method with an initial concentration of 20,000 ppm and an exposure of 4 hours. Outbred white rats of Wistar line of both sexes were used in the study, of which control and experimental groups were formed, including 6 individuals each (3 males and 3 females). The study was conducted under conditions of an inhalation chamber, in which nominal concentration of ozone and air mixture reached 20000 ppm, and the actual was 18000 ppm. The observation of the animals was carried out within 14 days after the test. During the treatment of animals with ozone and air mixture, a gradual suppression of the activity of respiratory system was observed with subsequent development of pulmonary edema and respiratory failure...