Changes in cell cycle of t-lymphocytes of patients with hemoblastosis after autologous grafting of peripheral stem hemopoietic cells

Changes in cell cycle and apoptosis in populations of CD4- and CD8- T-lymphocytes for patients with hemoblastosis before and after autologous grafting of peripheral stem hemopoietic ceBs (GPSHC) were analyzed. Apoptosis was shown can play a critical role in the limitation of CD4- T-cell recovery rate because the increase in proliferative activity was followed by the increase in apoptosis. The inverse correlation was observed for CD8-: the higher the proliferation the lower the apoptosis. The different correlation between proliferation and apoptosis most likely reflected the different rate of CD4- and CD8- T-lymphocytes recovery after grafting. Spontaneous apoptosis of CD8-T-lymphocytes 3 months after GPSHC was significantly lower in patients with unfavorable prognosis than in patients with remission. Patients with unfavorable prognosis demonstrated the reduction in the percentage of cells in SM phases for populations of both CD4- and CD8-T-lymphocytes. On the basis of data obtained it may be assumed that the spontaneous apoptosis and SM phases of cell cycle of T-lymphocytes are significant prognostic criteria for GPSHC efficiency.