Ecological conditions and taxonomic diversity of the microbial community of the Buksykhen cold spring water (Northern Pribaikalye)

The article studies the ecological living conditions of microbial communities of the Buksykhen spring. The analysis of physico-chemical parameters, macrocomponent and microcomponent composition by clustering has revealed the distribution of five outlets of the Buksyhen spring into two groups. For the first time, using molecular genetic methods, we have studied the taxonomic diversity of microbial communities of the five water outlets of the Buksykhen cold springs (Northern Pribaikalye). The analysis of molecular genetic studies has shown the dominance of representatives of Betaproteobacteria and Gammaproteobacteria classes of the Proteobacteria phylum in the water of the studied springs. A comparative analysis of family libraries has revealed that in all microbial communities, representatives of the Comamonadaceae family were dominant and codominant, the percentage of presence varied from 19 to 41%. The greatest differences in taxonomic composition were found in the microbial community of the Buksykhen-glaznoy (ophthalmic) outlet. The use of the SSA method allowed us to establish that the majority of families in the composition of the studied microbial communities are featured with the factors of Corg content, temperature, mineralization, pH-level and macrocomponents, which found to be significant