Kinetics of oxidase oxidation of ascorbic acid by horsеrsdish peroxidase

It is studied stationary kinetics reactions oxidations of an ascorbic acid, catalyzed by horseradish peroxidase. It is shown, that the ascorbic acid is slowly oxidized substratum peroxidase, with low constants of linkage of substrata of initiating concentration. In an interval pH 3,5-8,0 sizes kcat and Km are certain. In high concentration (80-240 juM) the ascorbic acid activates enzyme at all studied pH. The mechanism of action peroxidase in reactions oxidations of an ascorbic acid is offered.