Components of fractionated blood plasma and their role in the mechanism of reparative osteogenesis optimization

Purpose. To study the fractionated blood plasma in patients with limb bone fractures for some biologically active substances. Materials and Methods. Blood plasma fractionated according to an original technique studied for containing some biologically active substances in 10 patients with limb bone fractures in the postoperative period using immunoferment analysis. Results. TGF-β1, IGF-2, Ang-1 and -2, PDGF-AA, PDGF-BB, PDGF-AB, FGF-acidic, SCF, VEGF-А, sVEGFR1/Flt-1, TGF-α, EGF and MSP revealed to be contained in the concentration ranges of 10-6 -10-12 g/ml biologically significant for these compounds. The values of TGF-β2, FGFb and SCF-sR concentrations turned up to be below the sensitivity threshold of determination methods. The mechanism of participating biologically active molecules in reparative osteogenesis program proposed. Conclusion. More successful variant of regeneration may be determined not only by more competently made surgical manipulations, but also by the influence of biologically active components of fractionated blood plasma (growth factors, cytokines and their receptors) as participants of afferentation of bone tissue regeneration system.