Methods of molecular genetic diagnostics in the system of epidemiological surveillance in the hospital

Microorganisms that pose a threat of development of infections associated with healthcare (HAI) are present in every hospital. According to bacteriological tests, the level of colonization by nosocomial microorganisms of hospital facilities is from 5 to 36%, however, these tests do not always help to determine the pathways of infection. Additional information can be obtained from real time polymerase chain reaction (RT-PCR) tests, but their place in the system of epidemiological survey in the hospital has not yet been determined. Objective: to conduct a comparative study of bacteriological and RT-PCR tests results as part of epidemiological survey of HAI in the hospital. Materials and methods: 215 samples of washout from the objects of the hospital environment, as well as 53 samples of clinical material obtained from patients (loci: pharynx, nose, intestine) and employees (loci: pharynx, nose) were studied. Results and discussion: microbial contamination of objects in the hospital environment was 54...