Methods of sanitary surveillance of livestock production. V. Immune-enzyme analysis of sulfadimidine

Conjugates of sulfadimidine with albumin and gelatin, synthesized by azocoupling reaction were used for induction of rabbit antibodies and optimization of immune-enzyme analysis. The antibodies to conjugate with bovine serum albumin have a high specificity and do not interact with other sulfanilamides. The indirect solid phase competitive immune-enzyme analysis on the basis of obtained antibodies and immobilized conjugate with egg albumin permits to have the sensitivity of determination of sulfadimidine of 0.2 ng/ml. The possibility of application of this assay for control of residual content of sulfadimidine in milk, meat, eggs and the norms of its addition to food is discussed.