Characterization of mesenchymal stem cells isolated from feline and canine adipose tissue

Multipotent mesenchymal stem cells (MMSCs) are a promising tool of regenerative medicine for treatment of various small animal diseases. MMSCs have a high proliferative activity, multipotent properties, low immunogenicity, as well as the ability to migrate to the damaged tissue and promote its healing and regeneration. Currently, the methods of regenerative medicine are actively developing to solve problems that are difficult to cope with alternative treatments. However, data on the use of these cells in the clinic are ahead of the study of the properties of these cells in culture. This paper is our first report on isolation cells with phenotype similar to mammalian multipotent mesenchymal stem cells from feline and canine adipose tissue. The aim of the presented research was to study the cells properties with a phenotype similar to MMSC isolated from feline and canine adipose tissue (AT) in vitro. Isolation of cells was achieved by mechanical and enzymatic treatments of the AT...