Interspecific migration of agent brucellosis among yaks

The aim of the research is to study the presence of interspecifi migration of the pathogen of brucellosis among yaks and the specifi belonging of brucella using polymerase chain reaction. Laboratory studies were carried out using serological and classical methods of PCR analysis. The object of the study was yak farms and private farmsteads engaged in breeding yaks from the Issyk-Kul and Naryn regions, which have extensive high-mountain pastures. Blood from yaks of different sex and age was taken from a vein with a disposable needle into a sterile vacuum system of the Vacutainer type. At the fi stage, the presence of brucellosis infection in yaks was determined in the blood serum of animals using the rose-bengal test and enzyme-linked immunosorbent assay. Laboratory analysis of sera from 56 yaks indicated that in 14 cases they were infected with brucellosis. Clinical signs of disease manifestation during blood sampling did not appear. Then, positively reactive samples were examined by PCR to establish the type of Brucella. The results of laboratory tests showed that Br. Melitensis was found in the blood of infected yak, which indicated the ongoing migration of bacteria from small and large ruminants. The interspecifi migration of brucella was facilitated by the joint keeping of yaks with large and small ruminants on the common territory of farms and private households. The joint (small ruminants, large ruminants, yaks) year-round use of pastures of high-mountain natural pastures from the Naryn and Issyk-Kul regions also played an important role in the spread of brucellosis and brucella species migration. Determination of Brucella species belonging to a particular animal species will help to develop an evidence-based plan for anti-epizootic measures against brucellosis and select specifi means of prevention.