Microchip devices for polymeraze chain reaction. P. 1. PCR main principles; design and materials of microchips (review)

The main principles of polymeraze chain reaction (PCR) are considered. Features of the PCR homogeneous methods in real time are discussed. Questions of analytical signal formation during the reaction are stated. Advantages and lacks of PCR realization on a microchip are discussed. Devices for PCR with stationary and flowing reaction chambers are considered. General analysis of materials used in the construction of microchips for PCR is carried out. The main problems arising at use of the most widespread materials are considered.