Microfluidic device with y-shaped design for study of cell migration in concentration gradient of chemoattractants
Автор: Belousov Kirill Ilich, Bukatin A.S., Chubinskiy-nadezhdin V.I., Vasilyeva V.Y., Negulyaev Y.A., Evstrapov A.A., Kukhtevich I.V.
Журнал: Научное приборостроение @nauchnoe-priborostroenie
Рубрика: Приборостроение физико-химической биологии
Статья в выпуске: 1 т.26, 2016 года.
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Results of a development of microfluidic device for a study of cell migration under an influence of chemoattractant are discussed. Numerical simulation of chemoattractant distribution in various designs of microfluidic chip and estimations of the cells’ interaction with the liquid flow are conducted. Microfluidic chip design is chosen based on the simulation results. Microfluidic devices for experimental research are made by a soft lithography method in polydimethylsiloxane. The results of experiments carried out using CHO and HepG2 cells confirm the efficiency of the microfluidic chip and proposed approach.
Microfluidics, microfluidic device, cell migration, concentration gradient, computer simulation
Короткий адрес: https://sciup.org/14265006
IDR: 14265006
