Morphogenetic potential of explants Rhamnus L. genus representatives in vitro

The aim of the research was to choose phytohormonal content of nutritive environment under the conditions of in vitro culture to achieve the morphogenesis by explants Rhamnus alnifolia, R. diamantica, R. cathartica, R. imeretina and R. tinctoria. The material for the research were micro sprigs taken from three or five year old plants R. alnifolia, R. diamantica, R. cathartica, R. imeretina and R. tinctoria during the period of their active growth (10.05–20.06). Experimental research has been carried out in the laboratory of the microclonal propagation of plants of the National dendrological park “Sofyivka” of the National Academy of Sciences of Ukraine. The method of plants induction morphogenesis under the influence of growth regulation was used in species cultivation. Paravariation of nutrient solution Murasige and Skuga (MS) for morphogenesis induction has been carried out under the use of five tonicities of 6–benzylaminopurine (6-BAP) with addition of vitamins and amino acids. Under the influence of different tonicities of plant hormons the processes of morphogenesis took place in the most active way for R. alnifolia, R. diamantica, R. cathartica, R. imeretina and R. tinctoria in the environment IV under the tonicity of the nutrient solution of 6-BAP ― 2,0 mg/l, β-isobutyric acid — 1,0 mg/l, where the rate of reproduction during the second transfer comprised for R. diamantica — 5,3, and for R. tinctoria — 4,4, R. alnifolia — 3,5, R. cathartica — 2,0, R. imeretina —2,4.