New method of DNA melting signal treatment

The improved model of DNA melting signal is proposed in the article. The model is based on power polynomials of degree 3 (SP). Based on the model, a technique for processing the DNA melting signal has been developed, the main result of which is the determination of the DNA melting temperature Tm. The new technique allows to decrease the analysis time by increasing the temperature measurement pitch while simultaneously narrowing temperature range. Adequacy proofs of the model based on the SP are given. The application results of developed technique and of the known technique based on the sigmoid function (SF) are compared. The possibility of the technique using for determining the thermocycler tubes holder temperature distribution nonuniformity during melting mode is shown.