Evaluating the effectiveness of the management of mechanisms nephropathy with prolonged use of glucocorticoids

Theaim of this work was to study freeradical mechanism of nephropathy and to assess the capa-bilities of managing structural and functionalimpairmentin the kidneys of rats with glucocorticoid-induced oxidative stress by the antioxidant "Thiophane".The content and localization of proapoptotic protein factor Apaf-1 in the kidney parenchyma wasidentified by indirect immunohistochemical analysis carried out by the standard method. Blocking en-dogenous peroxidase was performed with 3% solution of hydrogen peroxide in the dewaxed sections. Specific monoclonal antibodiesto a protein APAF-1 (LabVision, 1: 100) were used as primary antibod-ies. The avidin-biotin complex (UltraVHRPpolymerKIT, LabVision) was used for labelling secondaryantibodies.It is shown that therenalinsufficiencyin the animalsreceiving long-term glucocorticoid, iscausedby damaging filtration apparatus components in the kidney glomerulus and by tubulointerstitialimpairment. It is found that the prolonged use of glucocorticoids leads to increased expression ofproapoptotic factors APAF-1 and lethal damage of nephrothelium by the apoptosis mechanism. Re-striction of free radical processes through the use of polyfunctional sulfur-containing antioxidant "Thi-ophane" reduces the development of structural and functional disorders in the renal glomeruli and thenumber of epithelial cells with signs of apoptosis in the tubules of the nephrons.