Evaluation of primers to highly effective PC genes using the Siberian oat genetic selection

Crown rust of oats, caused by the obligate parasite Puccinia coronata Corda f. sp. Avenae Erikss has a significant impact on oat production, leading to a decrease in grain yield and a deterioration in the quality of the resulting product. One of the most effective ways to combat crown rust epiphytoties is to create genetically resistant varieties. Molecular labeling methods can make this process more efficient and targeted. The purpose of research is to evaluate the effectiveness of primers for highly effective crown rust resistance genes using a genetic collection of Siberian oats. The material for the study was samples of oats cultivated by the Research Institute of Agriculture of the Northern Trans-Urals - a branch of the Tyumen Scientific Center SB RAS, as well as source varieties of highly effective genes for resistance to crown rust from the collection of the All-Russian Research Institute. To select optimal conditions for amplification, 3 different modes, 4 reaction mixtures, and a temperature gradient of 55.0-70.0 °C were used. The melting temperature was determined for each primer. Amplification products were separated in 2 % agarose and 8 % polyacrylamide gels. Amplification products were obtained, visualized on the gel in the form of clear bands. For none of the source varieties of Pc genes, amplification products were obtained that distinguished them from samples without these genes. This indicates their ineffectiveness for assessing the genetic collection of samples of Siberian selection. To successfully carry out marker-associated selection of oats for resistance to crown rust in Western Siberia, it is necessary to evaluate the effectiveness of other known primers for Pc genes, as well as the creation of our own marker systems.