The estimate of results of the real-time polymerase chain reaction of tumor markers cytokeratin-19 (CK-19) CDNA by electrophoresis method in microfluidic chip

In this paper the principal possibility of realization of the real-time polymerase chain reaction (RT-PCR) in microfluidic device was demonstrated. The rapid evaluation of the carried out PCR quality is an relevant objective. The possibility of this task solution by the electrophoresis of PCR products in microfluidic chip was represented. As the object of studying the cDNA of tumor marker cytokeratin-19 (CK-19) was selected. The methods of data treatment for informative electrophoretic signals were proposed and the conclusions regarding the quality of the PCR and the peculiarities of electrophoresis carrying out in microfluidic chip were made. The results of our researches can be used for the solution some perspective tasks, which also discussed in this paper.