Evaluation of free radicals level in the liver and kidneys of laboratory mice when exposed to short-term supercooling

This work has studied the effect of short-term supercooling on the organisms of laboratory mice. The effect of low temperatures leads to the activation of oxidative processes, which are characterized by a change in the level of free-radical metabolites of oxygen in the body. Thereby, we performed a bio-chemical luminescence analysis of homogenates of the tested tissues of laboratory animals subjected to short-term supercooling. It was done in order to estimate the level of free radicals, since the intensity of bio-chemical luminescence allows determining the rate of the reaction during which these radicals are formed. The intensity of chemical luminescence is directly proportional to the rate of radical formation, thus it becomes possible to evaluate the level of free radicals that are being formed in this system. The results of our study indicate that the level of oxygen free radicals in liver and kidneys increases when laboratory mice are exposed to short-term cold stress...