Optical clearing of eye tissues

In this work, we performed the estimation of diffusion coefficients of 40% aqueous glucose solution in both rabbit eye conjunctiva and sclera in vitro. The method is based on measurements of dynamics of the change of collimated transmittance of tissue samples under the action of biocompatible immersion liquid. The change of collimated transmittance is connected with matching of refractive indices of conjunctiva and sclera scatterers and interstitial fluid. The increase of tissue transparence was registered by consecutive recording of spectra of collimated transmittance in the range of 500 900 nm. For quantitative description of the process we have proposed a diffusion model, assuming the constant diffusion coefficient in the whole volume of the tissue sample. Estimation of diffusion coefficient was performed by approximation of experimental data within the framework of the proposed model.