Organogenesis of Kamchatka bilberry (Vaccinium praestans Lamb.) in clonal micropropagation

The results of studies on the cultivation of the Kamchatka bilberry (Vaccinium praestans Lamb.) of Sakhalin and Kuril forms in vitro at the stages of “proper micropropagation” and “rooting of microshoots” using a WPM nutrient medium with various content of micro and macro salts. 6-BAP cytokinin at concentrations of 0.5-1.0 mg/l, IBA and IAA auxins at concentrations of 1.0 and 2.0 mg/l are used as growth-regulating substances; solutions of Zircon preparations at a concentration of 0.5 ml/l and HB-101 at a concentration of 0.1 ml/l are used as biostimulants. The number of microshoots and roots of regenerated Kamchatka bilberry plants on the WPM 1/2 nutrient medium are significantly higher than in the variants with WPM and WPM 1/4. The maximum total length of microshoots (4.5-5.0 cm) of Kamchatka Bilberry is observed in the WPM 1/2 nutrient medium with 6-BAP at a concentrationof 0.5 mg/l and the addition of HB-101 at a concentration of 0.1 ml/l at the stage of “prorer micropropagation”. The maximum total length of roots (7.7-9.1 cm) of Kamchatka bilberryis observed on the WPM 1/2 nutrient medium with IBA at a concentration of 2.0 mg/l and the addition of HB-101 at a concentration of 0.1 ml/l at the stage of “rooting of microshoots”.