Optimisation of rt-pcr nucleic acid quantitative analysis
Автор: Belov Yu. V., Petrov A.I., Lavrov V.V., Kurochkin V.E.
Журнал: Научное приборостроение @nauchnoe-priborostroenie
Рубрика: Исследования, приборы, методики
Статья в выпуске: 1 т.21, 2011 года.
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The practical analysis of quantitative measurement errors was realized by RT-PCR at various methods of threshold cycle calculation. The threshold cycle values were obtained on analyzers of nucleic acids АНК-32 and АНК-64, developed in St.-Petersburg Institute for Analytical Instrumentation RAS. Effects of various errors in threshold cycle measurements are discussed. Recommendations on optimization of quantitative measurements are given.
Real-time pcr, dna, threshold cycle, quantitative analysis
Короткий адрес: https://sciup.org/14264699
IDR: 14264699
