Use of tiotriazoline for correction of morphological changes in rat’s prostate and seminal vesicles caused by exposure to epichlorhydrin vapor

The study aimed at determining the effect of tiotriazoline as a corrector of morphometric parameters of the prostate gland and seminal vesicles in immature rats. The experimental rats were exposed to epichlorohydrin inhalation. Materials and Methods. An experimental study was performed on 90 white male rats, which were introduced into the experiment at the age of 4 weeks, initial mass 40-50 g. The rats were divided into control and experimental groups. The control group consisted of intact rats. In the experimental group, rats were exposed to epichlorohydrin inhalation (10 mg/m3 during 60 days). In the second group rats, along with epichlorohydrin vapor, received tiotriazoline (117 mg/kg in abdominally) as a corrector. Linear size of seminal vesicles was determined using computer program for Morphometric Research “Master of Morphology”. The absolute and specific weights of the prostate and seminal vesicles were also determined. The author used hematoxylin and eosin for staining technique. Results. The experiment showed that 60-day epichlorohydrin inhalation in white immature rats resulted in a decrease in prostate gland and seminal vesicles weights. The author also observed a decrease in their linear size and destabilization of their structural and functional state after xenobiotic determination. Under the abovementioned conditions the use of cytoprotector tiotriazoline results in early recovery of the organ structure during the reconditioning period. The data were confirmed at the histological level. Conclusion. The use of tiotriazoline as a corrector of the morphological changes in the prostate gland and seminal vesicles along with epichlorohydrin leads to early recovery of the organ structures. It is proved by the decrease in the difference between the indices of intact rats and those exposed to toxin.