Prospects for the application of extracellular proteinases of micromycete Aspergillus ochraceus in the treatment of mastitis in cows

Cow mastitis, the etiological agents of which are pathogenic and opportunistic microorganisms, is considered one of the diseases that cause significant economic damage to dairy farming with a risk to the health of dairy consumers. With bacterial infections of the mammary gland in cows, the formation of protein exudates occurs. Proteinases that reduce the severity of the inflammatory response are included in the treatment regimens for various pathologies in medicine, but this practice is limited in veterinary medicine. In this work, we proved that a drug based on Aspergillus ochraceus proteinase increases the effectiveness of antibiotic therapy for cow mastitis. The aim of the study was to experimentally evaluate the possibility of using A. ochraceus BKM F-4104D micromycete proteinase in veterinary medicine. The studies were performed on lactating black-and-white cows ( Bos taurus ) with milk productivity for the previous lactation of 6900-7110 kg, which were divided into two groups ( n = 16 each). All experimental animals were intracisternally injected with an anti-mastitis drug based on the beta-lactam antibiotic amoxicillin, clavulonic acid and prednisolone (ACP) at a dose of 3.0 g (one syringe dispenser) once per day for 3-4 days until the disappearance of clinical signs of mastitis. In the second group, 12 hours before the use of the ACP preparation, animals were additionally intracisternally administered a drug with the working name PAO-1. PAO-1 is an oil suspension containing 4.0 g (syringe-dosing device) extracellular proteinase of the micromycete A. ochraceus BKM F-4104D as an active ingredient. This proteinase is able to degrade heterogeneous protein substrates in a wide range of environmental conditions, which can increase the effectiveness of the etiotropic therapy of bovine mastitis. The condition of the breast, morpho-biochemical status were evaluated before treatment, after treatment and 7-10 days after the end of the administration of the drugs. It was found that the combined use of an antimicrobial agent and a preparation based on the proteinase of the micromycete A. ochraceus BKM F-4104D was accompanied by the recovery of 93.8 % of cows with clinical mastitis, which is 12.5 % higher (p function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }