Polymerase chain reaction: devices and methods. A systematic review of literature

PCR-based analysis techniques and method are discussed. The focus of this paper is on the comparison of different methods of PCR implementation (e.g. classical PCR, qPCR and digital PCR) and their analytical characteristics (sensitivity, specificity, reproducibility and others). One of the most crucial tendencies of development PCR-based devices is microchip technologies. They can provide a high automation level of analytical manipulations and a high level of detection sensitivity. The mathematical models of PCR basic processes are presented in the paper. Also, we discuss some current methods of medical diagnostics.