Polymorphism of p53 gene-oncosupressor: age-specific features in risk of lung cancer development

Distribution of genotypes of p53 gene (duplication of 16 pairs of nucleotides at intron 3, Arg/Pro polymorphism at codon 72 of exon 4 and MspI-polymorphism at intron 6) in various age groups of lung cancer patients was studied. Assessment of p53 gene polymorphism was carried out using PCR-RFLP techniques. Risk of lung cancer development for patients aged younger 55 years was shown to associate with heterozygous genotypes at polymorphic sites of intron 3 and exon 4 as well as with the presence of unfavorable combination of heterozygotes at minor allel for all studied polymorphisms. No such associations were found in the studied group of patients with lung cancer. Distribution of genotypes significantly differed between patients of younger 55 years and older 55 years indicating the differences in risk factors for breast cancer of younger-aged patients as compared to older-aged patients.