Isolation of summer rape regenerants on selective media with nystatin and estimation their resistance to injurious insects

The order was developed for selection of summer rape regenerants on selective nutrient media with nystatin. Isolated plants-regenerants were tested on resistance to nystatin taking into account microcutting's striking root and frequency of callusogenesis. In the field and laboratory conditions the entomological estimation of tolerance of isolated rape plants to injurious insects - Pieris brassicae L. and Myzodes persicae Sulz. - was made. It was shown that nystatin resistance in substantial number of clones remains at level of undifferentiated cells and tissues. It was established that clones isolated on selective nutrient media with nystatin have increased resistance to Pieris brassicae L. Obtained results suggest about undirected action of nystatin.