Development and research of probiotic preparation for farm animals

Today, there is a high interest in the production of environmentally friendly and safe animal products. As a result, there is a growing demand for alternative feed additives that contribute to the productivity of farm animals and are aimed at maintaining the health of intestinal microflora. Such a valuable and safe alternative is the use of probiotic preparations in the diets of farm animals. Probiotics contain cultures of live microorganisms that are part of the normal microflora of the gastrointestinal tract of the animal. Probiotics have a general favourable effect on the body. In the production of probiotic supplements, the most commonly used microorganisms are lactobacilli (Lactobacillus plantarum) and propionic acid bacteria (Propionibacterium freudenreichii spp.). Lactobacilli are highly antagonistic and propionibacterium freudenreichii spp. are vitamin B12 producers. Deficiency of this vitamin in diets, negatively affects the physiological state and productivity of the animal. Three variants of experimental probiotic preparation for farm animals based on Lactobacillus plantarum and Propionibacterium freudenreichii spp. from the Siberian Collection of Microorganisms (SCM) of Federal Altai Scientific Centre of Agro-BioTechnologies were developed and studied. Two different cultivation methods were used in the production of the samples. The content of probiotic microorganisms in all tested samples was above the therapeutically important level and ranged from 1.43 x108 to 7.8 x108 CFU/cm3 for lactobacilli and from 3.73 x108 to 7.23 x108 CFU/cm3 for propionic acid bacteria. Vitamin B12 levels were quantified in all three samples. Vitamin B12 levels ranged from 0.7 to 0.82 mcg/ml.