Development and validation of liquid chromatography-tandem mass spectrometry (HPLS-MS-MS) method for detection of valproate in plasma

We developed the liquid chromatography-tandem mass spectrometry (HPLS-MS-MS) method for detection of valproate in blood plasma. Chromatography was performed on a reversed-phase Phenomenex synergi Fusion column (2 mm × 50 mm, 4 μm). The mobile phase consisted of 0.1% ammonium acetate in distilled water and 0.1% ammonium acetate in methanol (10:90% v/v, 0.5 mL/min). The multiple ions monitoring (MIM) mode was used for mass spectrometric detection of valproic acid. MS of valproic acid was m/z 143,1 in the negative ion mode. The quantitation limit of valproic acid in plasma was 0,5 mkg/ml. The range of this method was from 1 mkg/ml to 200 mkg/ml. The method was validated by the parameters of selectivity, accuracy, precision, linearity, cross-transfer, stability.