Development of an experimental version of quantitative PCR analysis software

This article presents the results of using the experimental version of the quantitative analysis software ANK_Cycles for the nucleic acid analyzers ANK-32, ANC-48 and ANK-96. Software was developed on the basis of a new method for automatic detection of the threshold cycle Ct. This method is based on approximation of the dependence of the Real Time Polymerase Chain Reaction (RT-PCR) signal by a polynomial of the 3rd degree. The experimental check of calibration error in quantitative PCR-analysis is carried out, and the results were compared with similar results obtained by the use of the known measurement technology (ANK_Shell software), which involves performing a number of manual operations. Measurements and comparisons were made in the fluorescence channels ROX and R6G with the use of samples of natural soybeans and genetically modified ones from the collection of reagents "Soybean GTS 40-3-2 quantity" produced by JSC "SINTOL" (Moscow).