Rhodotorula glutinis as a component of manure biofilm effluents

Analysis of manure biofilm effluents revealed an association of Clostridium ssp. (91.09 %), Escherichia coli (6.07), Bacteroides sp. (1.06), Sarcina ventriculi (0.91), Klebsiella sp. (0.14 %), and yeast Rhodotorula glutinis in the amount of 0.11 % of the total number of microorganisms. The pigmented yeast Rhodotorula glutinis has a low biochemical activity and is resistant to pH changes. In a natural environment (pH 7.0 ±0.3), the amount of Rhodotorula glutinis was 0.11% of the total number of micro- organisms (7*104±2.2 CFU/ml), but with a pH shift to 5.5±0.1 (acidification was carried out with 10 % sulfuric acid solution), the amount of yeast increased to 1.01 % (9*105±1.1 CFU/ml). Yeast of this species retains its viability when the temperature rises, so when heated to 70o C (in bioreactor plants when separating manure effluents), the amount of yeast was 0.06 % of the total microbial community (3*102±1.2 CFU/ml). The detection of yeast Rhodotorula glutinis in pig manure drains has not been described in the domestic and foreign literature before, their role requires further research.