Whole genome sequencing of Bacillus thuringiensis var. Darmstadiensis 56 strain and the study of insecticidal activity of the biological preparation on its basis

Multifunctional microbiological preparations are promising for use in plant protection due to their diverse effects including growth-promoting effect and complex antifungal and insecticidal activity. One of the key microorganisms used as the basis of biological preparations production is the gram-positive spore-forming bacterium Bacillus thuringiensis (Bt). The high specificity of the action and the environmental safety of Bt-based preparations contribute to maintain biocenosis balance and to reduce the number of treatments as well as to obtain environmentally friendly products. Previously, Bacillus thuringiensis var . darmstadiensis 56 (BtH10 56) strain was isolated and selected at the All-Russian Research Institute of Agricultural Microbiology. It possesses insecticidal effect for the larval stages of leaf-eating insect pests, growth-promoting activity for potatoes and antifungal effect against various phytopathogenic fungi. This paper presents the first data on sequencing and annotation of the whole genome of the BtH10 56 industrial strain; the factors responsible for the insecticidal and antifungal activity of this strain are identified, and the high efficiency of the biological preparation based on this strain is demonstrated under the field conditions against the Colorado potato beetle ( Leptinotarsa decemlineata Say...